Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Western Blot Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in untransfected HeLa whole cell extracts, but demonstrated a loss of signal in HeLa whole cell extracts transfected with siRNA. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in certain, normal human organ tissues. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).

Research Sub CategoryChromatin Biology

Detect the SNF2L protein using this Anti-SNF2L, clone SNF 2C4 validated for use in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleosome-remodeling factor subunit SNF2L, also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1 (SMARCA1), is the energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. These complexes facilitate the disruption of chromatin structure in an ATP-dependent manner. SNF2L potentiates neurite outgrowth, and may be involved in brain development by regulating En-1 and En-2 expression as well as in the development of luteal cells.

Immunogen

GST-tagged recombinant protein corresponding to human SNF2L.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected SNF2L in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~121 and ~123 kDa observed. Uniprot describes 5 tyrosine phosphorylation sites.